Potensi Antimikroba Protein Bioaktif dari Bakteri Simbion Alga Coklat Sargassum sp. Asal Perairan Pulau Lae-lae

????????????Penelitian untuk mempelajari potensi antimikroba fraksi protein dari bakteri Enterobacter agglomerans simbion alga coklat Sargassum binderi yang dikumpulkan dari Pulau Lae-lae, Sulawesi Selatan telah dilakukan. Senyawa Protein diisolasi menggunakan metode fraksinasi ammonium sulfat pada tingkat kejenuhan 0-20 %, 20-40 %, 40-60 % dan 60-80 %. Protein dimurnikan dengan metode dialisis menggunakan membran selofan. Uji aktivitas antimikroba terhadap bakteri patogen Escherchia coli dan Staphylococus aureus dengan metode difusi agar menggunakan paper disc. Hasil penelitian menunjukkan bahwa semua fraksi protein dari bakteri Enterobacter agglomerans yang diisolasi menunjukkan adanya senyawa protein bioaktif yang memiliki daya hambat terhadap pertumbuhan bakteri patogen. Fraksi protein 0-20 % memiliki daya hambat terbesar terhadap Escherichia coli dan Staphylococcus aureus dengan daya hambat masing-masing sebesar 10,00 mm dan 11,39mm

ANTIOXIDANT ACTIVITY AND TOXICITY POLYSACCHARIDE EXTRACT FROM RED ALGAE Eucheuma cottonii AND Eucheuma spinosum

Red algae Eucheuma cottonii and Eucheuma spinosum which one alternative source of antioxidant and anticancer because contains polysaccharide compound. This research aims to isolate and examine the activity of antioxidant and toxicity polysaccharide extract from red algae Eucheuma cottonii and Eucheuma spinosum . The study was carried out by isolating the polysaccharide extract using water and methanol-ethanol precipitation. Antioxidant activity of crude extract was examined using 1,1-Difenil-2-pikrilhidrazil (DPPH) method, and toxicity test was carried out using Brine Shrimp Lethality Test (BSLT). The research results showed that the crude extract polysaccharide for Eucheuma cottonii and Eucheuma spinosum have strong antioxidant activity with IC50 value of 72,49 ppm and 75,98 ppm. The result of BSLT assay showed that the crude extract polysaccharide has a highest toxicity with LC50 value of 165,88 ppm and 337,21 ppm there are classified as toxic. The crude extract polysaccharide Eucheuma cottonii and Eucheuma spinosum has a potential to be developed as an alternative antioxidant and anticancer agent

Essential oil constituents of Alpinia mutica Roxb.

The oil of Alpinia mutica Roxb was produced by hydrodistillation from fresh rhizomes, collected from cultivated plants in Universiti Pertanian Malaysia. The oil was analyzed by GC/MS and 24 components were identified which constituted 87.5% of the oil. The major components of the oil were camphor (35.6%), 1,8-cineole (9.4%) and borneol (8.3%)

Bioactivity of Protein Fraction in Brown Algae, Turbinaria decurrens, as Antibacterial Agent Bioaktivitas Fraksi Protein dalam Alga Coklat, Turbinaria Decurrens, sebagai agen anti bakteri

Penelitian ini bermaksud mengeksplorasi dan mengkarakterisasi fraksi protein dari alga cokelat Turbinaria decurrens di Sulawesi Selatan. Senyawa protein bioaktif diperoleh melalui serangkaian proses ekstraksi, fraksionasi dengan kejenuhan amonium sulfat 0-20%, 20-40%, 40-60%, dan 60-80% yang dilanjutkan dengan proses dialisis lalu ditentukan kadar proteinnya dengan metode Lowry. Fraksi protein yang diperoleh diuji bioaktivitasnya terhadap bakteri Staphylococcus aureus dan Salmonella thypi dengan metode difusi agar pada medium MHA. Hasil penelitian menunjukkan bahwa kadar protein tertinggi ditemukan pada fraksi 0-20% yaitu sebesar 5,1 mg/mL. Hasil uji bioaktivitas antibakteri terbesar ditemukan pada fraksi protein 0-20% terhadap Staphylococcus aureus dan Salmonella thypi dengan zona daerah hambatan masing-masing sebesar 18,33 mm dan 13,30 mm yang bersifat bakteriostatik. Hasil penelitian awal ini akan dikembangkan pada cakupan penelitian yang lebih luas untuk mengkloning protein bioaktifnya dan uji pre-klinis dari protein antibakteri yang diperoleh

Factors Influencing the Intention of Recipient to Pay Back Education Loan in State Foundation

This paper explores the factors that influence the intention to pay back the education loan among students in a state foundation. Education loan is the primary source for the student in order to pursue their studies. The government has introduced many forms of financial assistance such as scholarship, grants and education loan. However, the loan recipients have faced difficulties in repaying the loan, which will affect the provider and future recipients. This is because, the loan provider will not be able to sponsor future students if they are not able to collect the loan from the loan recipients. This paper identifies attitude, parental influence, media awareness and perception of loan agreement towards the intention to pay back the education loan. Theory of Planned Behavior (TPB) is suggested to be the underpinning theory of this study. Findings of the study can assist the state foundations on setting strategies to collect payment from the loan recipients

BIOACTIVITY OF PROTEIN FRACTION IN BROWN ALGAE, Turbinaria decurrens, AS ANTIBACTERIAL AGENT

A research on the ability of protein fraction from chocolate algae Turbinaria decurrens in territorial of South Sulawesi to inhibit the growth of Salmonella thypi and Staphylococcus aureus has been conducted. Proteins was fractionated from the whole extract using salting out method with 0-20%, 20-40%, 40-60% and 60-80% ammonium sulphate saturation. Protein was purified by a dialysis method using a selophan membrane. The protein level was determined by a Lowry method, the highest protein concentration, 5.1 mg/mL was found in the 0-20% fraction of the\ud chocolate algae. Antibacterial activity test was performed using a layered jelly diffusion method on the MHA medium. The highest antibacterial activity to Staphylococcus aureus was found in whole extract and the 0-20% fraction with the\ud inhibition diameter of 18.63 mm and 13.30 mm, respectively

BIOAKTIVITAS ANTIBAKTERI FRAKSI PROTEIN ALGA MERAH Gelidium amansii DARI PERAIRAN CIKOANG KABUPATEN TAKALAR SULAWESI SELATAN

A research on the ability of protein fraction from red algae Gelidium amansii to inhibit the growth of Salmonella thypi and Staphylococcus aureus has been conducted. Proteins was fractionated from the crude extract using salting out method with 0-20%, 20-40%, 40-60% and 60-80% ammonium sulfat saturation. Proteins was purified by a dialysis method using a selophan membrane. The protein level was determined by a Lowry method,\ud the highest protein concentration, 5.6 mg/mL was found in the 40-60% fraction of the red algae. Antibacterial activity test was performed using a layered jelly diffusion method on the MHA medium. Results indicated that crude extract and the protein fraction of the red algae had antibacterial effect to Salmonella thypi and Staphylococcus aureus. The highest antibacterial activity to Staphylococcus aureus was found in the 0-20% fraction with the\ud inhibition diameter of 14.43 mm after incubated for 24 hours. These research might provide a significant results for the following research on the antibacterial action in molecular level of protein compounds from marine algae

PENGARUH MEDIA SOSIAL TERHADAP MINAT BACA GENERASI Z (SURVEY PADA FOLLOWERS AKUN TWITTER MEDIA @CNNINDONESIA)

Social media is one of many technology developments which already common in human’s life, especially for generation Z whom half of the daily works are done with the help of social media. Social media is stated as one factor that influence the development of children’s behaviour, also to human’s reading interest. In the certain amount of frequency and quality of use of social media is considered capable of influencing one’s reading interest and reading quality. This research was aimed to find out the influence of social media towards generation Z’s reading interest. The sample used in this research was generation Z whom followers of one media company, CNN Indonesia (@CNNIndonesia) on twitter with purposive sampling technique. Keywords: Social Media, Reading Interest, Generation

PRODUCTIONAND CHARACTERIZATIONOF CHITINASE ENZYMESFROMSULILI HOT SPRING IN SOUTH SULAWESI, Bacillus sp. HSA,3-1a

ABSTRACT Chitinase is an extracellular enzyme which is capablfJin hydrolyzing insoluble chitin to its oligomeric and monomeric components. The enzyme produced by thermophilic bacteria was screened and isolated from Sulili hot spring in Pinrang, South Sulawesi, Indonesia. The gram positive spore forming rod shape bacteria was identified as Bacillus sp. HSA,3-1a through morphological and physiological analysis. The production of chitinase enzyme was conducted at various concentration of colloidal chitin at a pH of 7.0 and a temperature of 55°C. The bacteria optimally was produced the enzyme at a colloidal chitin concehtration of 0.5% after 72 h of incubation. The optimum temperature,pH and substrate concentration of chitinase were 60°C, 7.0 and 0.3%, respectively. The enzyme was stable at a pH of 7.0 and a temperature of 60 °C after 2 h of incubation. The chitinase activities was increased by addition of 1 mM MI+, Ca2+and Mn2+ions, whereas the activities were decreasedby 1mM C02+,Fe2+and Zn2ions.The molecular weight of the crude enzyme was determined using SOS-PAGEanalysis. Five protein fractions were obtained from SOS-PAGE,with MWs of 79, 71, 48, 43 and 22 kOa. Keywords: colloidal chitin, thermophilicbacteria, chitinas